One of the challenging aspects of conducting anticoccidial studies where the treatment is incorporated into feed is obtaining an accurate assessment of the incorporation of treatment by feed analysis.
Vetspin work with a culture of “continual improvement” in how they operate and think. To that end the company has recently developed an in house method for analysis of coccidiostats in poultry feed. This method has been validated under GLP so to now offer the service to the Sponsors studying the effectiveness of coccidiostats in studies. The method is based on HPLC-MS/MS after extraction and dilution of the sample.
The results obtained show good consistency within and between runs and low uncertainty and limits of detection, providing the necessary confidence that the methods that have been developed are fit for checking coccidiostat concentration in feed.
It should be noted that the present regulations governing feed analysis for regulatory studies are very prescriptive and do not exactly match the methods developed by Vetspin. They are specified in:
Whilst Vetspin’s methods do not conform to these methodologies they believe that they are an improvement on the prescribed methods and aligned with the recent literature. Vetspin is now offering these improved methodologies to Sponsors and hope that EU regulators raise their awareness of these new methodologies.
Vetspin prides itself in not just operating to best industry standards but importantly also contributing to operating improvements within animal health and specifically drug/chemical analysis.
If you wish to find out more on what Vetspin is doing and whether they can assist your with your development programme, please contact them on https://www.vetspin.com/contacts.
References
Moretti et al.: Development and Validation of a Multiresidue Liquid Chromatography/Tandem Mass Spectrometry Method for 11 Coccidiostats in Feed; Journal of AOAC international Vol. 96, no. 6, 2013
Vincent et al.: Determination of ionophore coccidiostats in feedingstuffs by liquid chromatography–tandem mass spectrometry Part I. Application to targeted feed; Journal of Pharmaceutical and Biomedical Analysis 47 (2008) 750–757